The three related sign languages, Japanese Sign Language (JSL), Korean Sign Language and Taiwan Sign Language, are reported to have a gender distinction in pronouns and other signs. For historical reasons and because of an emblem for "female' in the hearing Japanese culture, the distinction is taken to originate in JSL. In this chapter we give examples of the three relevant markers: +FEMALE (a fist with the little finger extended), +MALE (a fist with the thumb extended), and +FEMALE+MALE (a fist with the little finger and the thumb extended). We exemplify the different uses of the three markers in one monologue and two dialogues in JSL and discuss their uses in signs with a referential function, signs with a predicational function, and in depicting constructions. Most instances of the gender markers in the data are found in lexical nouns and a few lexical verbs. We also found cases with uses of the gender markers in signs whose function as either referential or predicational is unclear. Some signs start out as nominals with a referential function and then develop into depicting constructions which denote the referents' motion or being in a place. A further use of the markers is in constructions where signers refer to a referent by pointing to the articulators of the markers. We suggest that the versatility of the markers is due to the fact that the spatial medium and gestural modality permit sign languages to represent referents by means of articulators, including the articulators of the gender markers.